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. 2015 Apr 30;5:9878. doi: 10.1038/srep09878

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(A) Ribbon representation of the overall structure of MRPP3-C and PRORP1. For MRPP3-C, the PPR domain, the center domain and the metallonuclease domain are colored purple, green and gold, respectively. (B) in vitro cleavage assays with the human mitochondrial tRNA^Ile precursor. (C) in vitro assays with 5 mM Mg²⁺, Mn²⁺ or EDTA. (D) Purity of the proteins used in the in vitro cleavage assays. Full-length gels are presented in Supplementary Figure S2.