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Concentration-dependent effects of CQ on HCT15 cells. (A) HCT15 cell lines were treated with various concentrations of CQ and CQ plus BEZ235 (5 nM) for 48 hours. Cell viability was analyzed by MTT assay. (B) HCT15 cells were treated with 0, 10 and 100 uM of CQ for 24 hours, stained with 1 ug/mL acridine orange and then observed under fluorescence microscope. All experiments were repeated three times. Bar, 10 um. Error bars, SEM. *, p<0.05.
