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. 2014 Dec;18(4):225–231. doi: 10.12717/DR.2014.18.4.225

Fig. 3.

Fig. 3.

Antioxidants increase the cell viability and ROS is produced following CQ treatment. (A) HCT15 cells were treated with various concentrations of CQ plus DMSO, 10 mM NAC and 500 uM trolox. Cell viability was analyzed by MTT assay. (B) HCT 15 cells were treated with 0, 10 and 100 uM of CQ for 24 hours. Cellular ROS production was examined with the Image-iT Live Green ROS Detection kit and visualized under fluorescence microscope. Experiments were performed in triplicate. Bar, 10 um. Error bars, SEM. *, p<0.05, **, p <0.01.