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Nucleosome pull-down. A) Pull-down of LEDGF from crude nuclear extract using nucleosomes bearing wide type (WT) H3, MLA (H3KC36me3) or H3K36me3, as well as peptide bearing H3K36me3. The blot is imaged with LEDGF antibody or Direct Blue-71 (DB-71) staining of total protein. B) Silver-stain for the total protein pulled down by H3K36me3-containing and wild-type nucleosomes from partially fractionated HeLa nuclear extract.
