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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Methods Mol Biol. 2015;1271:173–185. doi: 10.1007/978-1-4939-2330-4_12

Fig. 1.

Fig. 1

(A) An AFM deflection image of a ROS disc membrane adsorbed on mica. Scale bar, 500 nm. (B) Illustration of a SMFS experiment. The AFM probe attached to a flexible cantilever is non-specifically bound to the N-terminal end of rhodopsin embedded in ROS disc membranes. As the AFM probe is retracted from the sample surface, rhodopsin is mechanically unfolded in a stepwise manner. The stable structural segments of rhodopsin are highlighted in different colors. This figure is reproduced from (13) with permission from the American Society for Biochemistry and Molecular Biology.