Table 2.
Fatty acid composition of control and omega-3 PUFA diets.
| Fatty acids | Control diet (mg/g) | EPAX diet (mg/g) |
|---|---|---|
| C14:0 | 0.4 | 0.4 |
| C16:0 | 5.1 | 2.1 |
| C18:0 | 3.9 | 1.7 |
| C18:1 | 18.5 | 9.1 |
| C18:2n-6 | 21.3 | 11.2 |
| C18:3n-3 | 0.83 | 0.5 |
| C20:4n-6 (AA) | ND | 0.9 |
| C20:5n-3 (EPA) | ND | 22.2 |
| C22:6n-3 (DHA) | ND | 2.0 |
| Total fatty acids | 50.0 | 50.0 |
| ∑n-6 PUFA | 21.30 | 12.06 |
| ∑n-3 PUFA | 0.83 | 24.59 |
| (n-6)/(n-3) | 25.80 | 0.49 |
| (n-3)/(n-6) | 0.04 | 2.04 |
| ∑SFA | 9.40 | 4.26 |
| ∑PUFA | 22.13 | 36.65 |
| ∑MUFA | 18.50 | 9.07 |
| PUFA/SFA | 2.35 | 8.60 |
The chemical composition of control diet was as follows (g/kg dry diet): starch, 587; casein, 200; cellulose, 50; sucrose, 50; mineral mix, 40; vitamin mix, 20; DL-methionine, 3; vegetable oil-Isio-4 (Lesieur, Neuilly-sur-Seine, France), 50. Total oil represented 5% of the diet. In the omega-3 PUFA diet, half of the vegetable oil-Isio-4 was replaced by EPAX-7010 (the omega-3 PUFA oil). The vegetable Isio-4 oil contained the following: 47.2 mg/g 18:2 (n-6); 1.7 mg/g total (n-3); and 40.2 mg/g monounsaturated fatty acids (largely 18:1). EPAX-7010 oil, in the form of ethyl ester, contained approximately 85% (n-3) PUFA, that is, EPA, 70%, DHA, 12%, and α-tocopherol, 2.1 to 3.2%. It means that EPAX oil represented 2.5% of the diet. Since the omega-3 PUFA consisted of 85% of the 2.5% EPAX oil, the total n-3 PUFA represented only 2.1% of the total diet. After diets' preparation, the lipids from diets were extracted according to the method described in Yessoufou et al., 2006 [27], and then transmethylated by BF3/methanol after saponification, and fatty acids were analysed by gas liquid chromatography.