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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Virology. 2014 May 24;0:190–208. doi: 10.1016/j.virol.2014.03.021

Figure 9. The sf-HCVcc density profile was maintained afterin vitro incubation with serum.

Figure 9

The sf-SA13(5a) recombinant was mixed 1:1 with either 100% FBS, DMEM + 10% FBS, DMEM, AEM, 100% human serum or sterile filtered cell culture conditioned medium (DMEM + 10% FBS harvested after 48 hours culture on naïve Huh7.5 cells) and incubated for 6 hours at 37°C. Mixes were layered on top of a pre-formed 10–40% iodixanol gradient, and subjected to ultracentrifugation as described in Materials and Methods. Fractions were collected from the bottom of the gradients and analyzed by infectivity titration and by density determination as described in Materials and Methods. Relative recovery per fraction (%) was calculated by relating the amount of infectious virus detected in each fraction to the total amount of infectious virus collected, and is plotted against the density determined for each fraction.