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. 2015 Apr 30;11(4):e1005119. doi: 10.1371/journal.pgen.1005119

Fig 5. Nucleotidyl transferase assays to evaluate the substrate preferences by HESO1 and URT1.

Fig 5

Reactions were conducted with HESO1 (A) or URT1 (B) and five RNA oligonucleotides (oligo) in a time course as indicated. The sizes of the input miRNAs are indicated (19 nt for miR158-1 and 20 nt for the full-length forms of miR158). The RNAs were 5’ 32P-labeled and gel purified prior to the reactions. The substrate/enzyme ratio was 1:2 for HESO1 and 1:8 for URT1. The sizes of the RNA substrates are indicated.