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. 2015 Apr 30;11(4):e1004863. doi: 10.1371/journal.ppat.1004863

Fig 7. IFIT1 inhibits replication of alphaviruses in a concentration-dependent mode.

Fig 7

(A) The schematic representation of the genomes of alphaviruses used in the experiments presented in this figure. (B) Populations of BlaR, IFIT1-expressing and parental cells were used for standard plaque assay of VEEV/GFP and SINV/GFP viruses. Since both viruses were unable to induce plaque formation on stable IFIT1-expressing cells, foci of GFP-expressing cells were detected on a Typhoon phosphorimager. (C) The results of RT-qPCR analysis of IFIT1-specific mRNA in different clones of IFIT1 KI cells, derived from wt MEFs, which were used in the experiments presented on the next panel and in the following sections. (D) The indicated clones of IFIT1 KI cells were infected at an MOI of 1 PFU/cell with VEEV/GFP. Media were replaced at the indicated time points, and virus titers were determined by plaque assay on BHK-21 cells.