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. 2015 Apr 30;10(4):e0123441. doi: 10.1371/journal.pone.0123441

Fig 10. Association of REH2 with components of its MRB requires normal conserved domains.

Fig 10

(A) REH2 (2,167 amino acids) includes the double-stranded RNA binding (dsRBD) and DExH-helicase domains, and an OB-fold domain not previously identified. Expressed TAP-tagged REH2 variants in Dynabead IgG pulldowns were tested for (B) gRNA in radiolabeled capping assays of the 5’ triphosphate, (C) fold enrichment of edited mRNA at block 1 or at a distal block, and unedited pre-mRNA. RT-qPCR assays were normalized to values in the untagged REH2 pulldown set at 1. Tubulin and 18S rRNA carryover were used as reference, (D) GAP1 and REH2 in western blots. Cells-/+ Tet induction of wild-type (WT), mutants, or untagged REH2 (no-Tag) were compared. Standard deviation of the average value in Cq duplicates is shown.