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. 2015 Apr 30;10(4):e0124887. doi: 10.1371/journal.pone.0124887

Fig 2. Subcellular localization of four AtBolA proteins and tissue specific expression of AtBolA3 in Arabidopsis thaliana.

Fig 2

(A) Subcellular localization of BolA1, BolA2, BolA3 and BolA4 in Arabidopsis protoplasts, which were transiently transformed with BolA-GFP constructs. CHL: chloroplast; BR: bright field; Merged: GFP and CHL overlay. (B) Spatial expression pattern of AtBolA3prom::GUS in one-week-old seedlings under normal growth condition. (C) Expression of AtBolA3 detected by qRT-PCR in various tissues of Arabidopsis thaliana at the vegetative and reproductive stages. The relative expression was calculated as the ratio of the expression of AtBolA3 to that of housekeeping gene, AtUBQ11, with four independent biological replicates. VL and VR: leaf and root in the vegetative stage; YL, OL, SL, R, F, P, and S stand for young leaf, rosette leaf, stem leaf, root, flower, pod, seed in the reproductive stage. DS: dry seed.