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. 2015 Apr 30;10(4):e0122679. doi: 10.1371/journal.pone.0122679

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© 2015 Shi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) The expression level of p21 mRNA was measured using qRT-PCR. (B, C) Analysis of p21 expression in sw480 and HCT116 cells treated with pCDNA-BANCR by Western blot. The results are from three independent experiments. GAPDH protein expression was used as an internal control. (D) The IHC showed an upregulation of BANCR increased p21 expression in a xenograft mouse model. (E F) The MTT assay was performed to determine the proliferation of SW480 and HCT116 cells transfected with empty vector, pcDNA-BANCR+p21-shRNA or pcDNA-BANCR. Data represent the mean ± S.D. from three independent experiments. *, P < 0.05.