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. 2015 May 1;8(5):429–441. doi: 10.1242/dmm.018630

Fig. 1.

Fig. 1.

Migration of neural crest is required for expression of noradrenergic markers in the anteroventral region. Xenopus embryos were injected in one cell at the two-cell stage with β-galactosidase (light blue; A) as a tracer along with either GFP as a control, or mRNA encoding a dominant-negative form of Slug (dnSlug) to inhibit neural crest migration; cells derived from the injected cell are shown in the side to the right in A. (A) Embryos were fixed at stage 17/18 and subject to in situ hybridisation for the neural crest markers Slug, Snail and FoxD3, and noradrenergic (NA) markers Phox2a and Hand2. (B) Embryos were scored compared to the uninjected side based on inhibition of migration for neural crest markers and reduced expression of NA markers (see supplementary material Fig. S3). For scoring data, n=28-45. Kruskal–Wallis non-parametric ANOVA was performed, comparing GFP control with dnSlug-injected embryos (**P<0.0005 and ***P<0.000005).