Fig. 6.

CDK activity inhibits AVNA cell differentiation induced by WT ASCL1 but not S-A ASCL1. Xenopus embryos were injected in one cell at the two-cell stage with β-galactosidase (light blue; A) as a tracer along with mRNA encoding cyclin A/CDK2 and/or mouse WT/S-A Ascl1, as indicated; cells derived from the injected cell are shown in the side to the right in A. (A) Embryos were subject to in situ hybridisation for AVNA markers at stage 18-19, as labelled. (B) Embryos were scored for marker expression comparing the injected and uninjected side (see supplementary material Fig. S3 for scoring scheme). For scoring data, n=20-32. Kruskal–Wallis non-parametric ANOVA was performed comparing WT ASCL1 with S-A ASCL1 without and with cyclin A/CDK2 (*P<0.05 and ***P<0.000005).