Fig. 7.

N-Myc inhibits AVNA cell differentiation induced by WT ASCL1 but not S-A ASCL1. Xenopus embryos were injected in one cell at the two-cell stage with β-galactosidase (light blue; A) as a tracer along with mRNA encoding N-Myc with or without mouse WT/S-A Ascl1, as indicated. (A) Embryos were subject to in situ hybridisation for AVNA markers as indicated at stage 18-19. (B) Embryos were scored for marker expression comparing the injected and uninjected side (see supplementary material Fig. S3 for scoring scheme). For scoring data, n=24-32. Kruskal–Wallis non-parametric ANOVA was performed comparing WT ASCL1 and S-A ASCL1 with and without N-Myc (*P<0.05, **P<0.0005 and ***P<0.000005).