Fig. 2.

VPA treatment increase Pygb expression in mice skeletal muscle cultures. (A) After myotubes were formed, muscle cultures were treated with different concentrations of VPA for 72 h. (B) In both cell cultures (WT and KI), Pygb mRNA increased in treated cultures (**P<0.01, *P<0.05). (C) GP-BB (Pygb) protein was also detected with western blot analysis in WT and in KI cell cultures. Abbreviations: DM, Dulbecco's modified Eagle's medium; KI, knock-in; VPA, valproic acid; WT, wild type; RQ, relative quantification.