Skip to main content
. 2015 Apr 30;10(4):e0125377. doi: 10.1371/journal.pone.0125377

Fig 4. GAPDH binds to the peptidoglycan.

Fig 4

A quantity of 100 μg of cell wall suspensions containing peptidoglycan and the teichoic acids (PG+TA) or only peptidoglycan (PG) as well as purified lipoteichoic acids (LTA) were coated on 96-well plate and incubated with GAPDH and the C-reactive protein (CRP). After extensive washes, bound proteins were immunodetected using anti-GAPDH and anti-CRP sera. Chemiluminescence was measured after extensive washes and expressed as arbitrary units. Each assay was performed three times in triplicate wells. A representative experiment is shown.