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. 2015 Apr 30;10(4):e0125065. doi: 10.1371/journal.pone.0125065

Fig 3. Photomicrographs of representative lung sections obtained from C57BL/6 mice after a single bleomycin instillation (stained by picrofuchsin).

Fig 3

Tissues were stained with picrofuchsin to determine the collagen content (21st day of the experiment). (A) Mice receiving intratracheal 0.9% NaCl; (B) Mice receiving intratracheal BLM; (C) Mice with fibrosis spiperone treated; (D) Mice with fibrosis HYAL treated; (E) Mice with fibrosis pegHYAL treated; (F) Mice with fibrosis pegHYAL and spiperone treated. Dark pink stains are collagenous deposits. The most expressed collagen fibers deposition after BLM injection was observed on the 21st day. The photomicrographs were taken using an Axio Lab.A1 (Carl Zeiss MicroImaging GmbH; Göttingen, Germany) microscope and AxioCam ERc5s digital camera. All photomicrographs were at 100 × magnification.