Table 1. Effects of hyaluronidase (HYAL) and Spiperone treatment on the content of the connective tissue in the lungs of C57BL/6 mice at the partially reversible pneumofibrosis (% of the area of lung tissue).

Groups	Day after single dose bleomycin instillation
	7	14	21
Mice received intratracheal 0.9% NaCl	1.13±0.07	1.14±0.03	1.15±0.06
Mice with fibrosis 0.9% NaCl treated	3.07±0.09*	3.91±0.07*	4.26±0.14*
Mice with fibrosis HYAL treated	3.05±0.17*	2.60±0.21* &	3.24±0.07*
Mice with fibrosis pegHYAL treated	2.22±0.12* &	2.12±0.12* &	2.24±0.18* &
Mice with fibrosis Spiperone treated	2.09±0.15* &	2.75±0.12* &	2.12±0.13* &
Mice with fibrosis pegHYAL and Spiperone treated	1.45±0.08 &	1.22±0.14&	1.40±0.09&

The content of collagen fibers in lung was determined using a function for counting the area of the object in the image. Broncho-vascular strands were carefully removed from the analyzed areas. The relative fibrotic tissue area is calculated of by the formula: $X=\Sigma ax100/\left(S-\Sigma b\right)$ where: a—is the amount of pixels occupied by fibrotic tissue in 10 pictures of one drug, S—is the number of pixels corresponding to the total area of the image (using this camera and software—4,423,680), b—a sum of pixels occupied by the empty part of the glass slide in 10 pictures of one drug.

Data represent mean of 3 independent experiments, n = 10/group. Results are presented as mean and SEM.

*—compared to the mice received intratracheal 0.9% NaCl (P <0.05),

&—compared to the mice received intratracheal BLM and 0.9% NaCl treated (P<0.05) t test was used.