Table 2. Effects of hyaluronidase (HYAL) and Spiperone treatment on the content of the connective tissue in the lungs of C57BL/6 mice at the irreversible pneumofibrosis (% of the area of lung tissue).

Groups	Day after bleomycin instillation
	21	40	60
Mice received intratracheal 0.9% NaCl	1.15±0.06	1.12±0.18	1.23±0.16
Mice with fibrosis 0.9% NaCl treated	5.13±0.75*	4.12±0.40*	5.96±0.93*
Mice with fibrosis HYAL treated	2.84±0.32* &	2.61±0.41*&	5.90±0.79*
Mice with fibrosis pegHYAL treated	1.87±0.19* &	2.09±0.19*&	4.78±0.64*
Mice with fibrosis Spiperone treated	1.86±0.15* &	1.52±0.13*&	4.96±0.31*
Mice with fibrosis pegHYAL and Spiperone treated	1.05±0.09 &	1.22±0.11 &	2.78±0.19*&

The content of collagen fibers in lung was determined using a function for counting the area of the object in the image. Broncho-vascular strands were carefully removed from the analyzed areas. Data represent mean of 3 independent experiments, n = 10/group. Results are presented as mean and SEM.

*—compared to the mice received intratracheal 0.9% NaCl (P <0.05),

&—compared to the mice received intratracheal BLM and 0.9% NaCl treated (P<0.05), t test was used.