Table 3. Effects of hyaluronidase (HYAL) and Spiperone treatment on collagen type I, hydroxyproline, total soluble collagen, hyaluronic acid levels after the single dose intratracheal bleomycin instillation.
| Groups | Collagen type I | Hydroxyproline | Total soluble collagen | Hyaluronic acid |
|---|---|---|---|---|
| Mice received intratracheal 0.9% NaCl | 121.1±11.7 | 2915±240 | 69.6±6.3 | 28054±1901 |
| Mice with fibrosis 0.9% NaCl treated | 233.3±22.4* | 8714±651* | 127.2±11.4* | 98948±3358* |
| Mice with fibrosis HYAL treated | 194.6±17.3* | 5661±402● | 99.3±8.1 | 98611±7754* |
| Mice with fibrosis pegHYAL treated | 188.9±15.4 | 4303±321● | 83.05±5.5● | 34736±2604● |
| Mice with fibrosis Spiperone treated | 156.8±12.1● | 5693±298● | 101.2±6.1● | 43086±2857* ● |
| Mice with fibrosis pegHYAL and Spiperone treated | 164.6±11.1● | 3486±287● | 74.1±7.2● | 32649±1921● |
Hydroxyproline, collagen type I, total soluble collagen and hyaluronic acid levels were measured in homogenate of right lung lobes from C57BL/6 mice on 21st day after BLM treatment. Hydroxyproline, collagen type I and HA were assayed by ELISA according to manufacturer instructions (Cusabio Biotech CO., Ltd, China). The right lung lobes were excised and snap frozen after having measured the wet weight. Sensitivities were >1.95 ng/mL for hydroxyproline, >0.039 ng/mL for collagen type I and >15.6 pg/mL for HA. The total soluble collagen was determined using a standard curve for the SircolTM assay (Biocolor Ltd, UK) according to manufacturer’s instructions. Results were expressed as μg collagen per right lung.
Data represent mean of 3 independent experiments, n = 5/group. Results are presented as mean and SEM.
*—compared to the mice received intratracheal 0.9% NaCl (P <0.05),
●—compared to the mice received intratracheal BLM and 0.9% NaCl treated (P<0.05), t test was used.