Fig. 4.

Activation of ERK1/2 was inhibited in Hep3B cells by the combination of 5-FU and CGA. Hep3B cells were treated as shown in Fig. 1 for 24 h. (a) Cell lysates were prepared and subjected to immunoblot analysis to determine the level of total ERK1/2 and phospho-ERK1/2. (b) The intensity of the band was quantified using densitometric imaging. Experiments were conducted in triplicate. Data are shown as the mean±SEM (n=3). *P<0.05 compared with the control. CGA, chlorogenic acid; ERK, extracellular signal-regulated kinase; 5-FU, 5-fluorouracil.