Table 1. Promoter analysis of differentially expressed defense-related genes in WRKY70 transgenic plants.
| Motif | Background |
Upregulated cluster |
Downregulated cluster |
F | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Occ | Avg | Stdev | Occ | Avg | Stdev | Occ | Avg | Stdev | ||
| TTTGAC[CT] | 13 781 | 10.72 | 3.37 | 13 | 10.49 | 3.05 | 6 | 12.03 | 2.75 | 73.20 |
| TGAC | 28 073 | 131.40 | 12.49 | 24 | 151.86 | 12.12 | 10 | 146.03 | 10.50 | 806.73 |
| TTGAC[AC] | 23 170 | 27.26 | 5.43 | 23 | 31.98 | 6.12 | 9 | 22.41 | 3.12 | 897.09 |
| TTGACA | 18 860 | 16.99 | 4.20 | 16 | 20.06 | 5.66 | 6 | 10.43 | 2.42 | 1307.79 |
| TTGAC[CT] | 22 721 | 26.59 | 5.64 | 21 | 41.30 | 8.17 | 9 | 34.64 | 4.91 | 1327.08 |
| TGAC[CT] | 27 594 | 65.61 | 8.90 | 24 | 87.97 | 9.96 | 10 | 79.41 | 9.33 | 1439.90 |
| TTGAC[ACT] | 26 243 | 43.94 | 7.39 | 24 | 61.31 | 9.01 | 9 | 45.24 | 5.62 | 1678.35 |
| TTGAC[AC]A | 14 413 | 10.82 | 3.44 | 8 | 6.92 | 3.00 | 7 | 15.08 | 2.91 | 1709.38 |
| TTGAC | 26 904 | 51.08 | 7.85 | 24 | 76.70 | 9.73 | 10 | 57.15 | 5.10 | 2947.19 |
| TTGAC[AC][CGT] | 18 094 | 16.10 | 4.18 | 19 | 25.16 | 5.10 | 3 | 7.49 | 3.04 | 4443.95 |
The background contains all 28 088 genes from A.thaliana, the upregulated cluster contains 24 genes and the downregulated cluster contains 10 genes (25). All analysed promoter sequences were 1500 bp long and POBO was run with the following parameters: number of pseudoclusters 1000, number of promoters in a pseudocluster 15 and length of the background promoters 1500 bp. Occ is the number of promoters containing the given motif, Avg is the mean number of motifs in a cluster, Stdev is standard deviation of the mean and F is the F-value from ANOVA.