Fig 12. MR5057-miR-3p interacts with the 3′ UTR of CyHV-3 ORF123 and downregulates its encoded protein.

(A) Western blot showing relative CyHV-3 rdUTPase levels (top panel) produced 48 h following transfection of CCB cells with pCyHV-3-rdUTPase in combination with miRNA mimics (as indicated above the image). Endogenous β-actin levels are also shown from the same blot (lower panel). (B) Luciferase assays showing relative values obtained from pLS-123-3′ UTR (containing predicted target seed region 5′GCAATT3′) and matched control pLS-123-3′ UTRmut (seed changed to 5′CGTACA3′) in the presence of miRNA mimics. Data are presented as the Log₂ ratios of luciferase activities of targeting to non-targeting miRNA mimics (i.e. MR5057-miR-3p to negative control). The data shown were compiled from three experiments, values represent means between experiments and error bars represent standard deviations; *indicates p < 0.05.