Fig. 4.

Immunohistochemical analysis of the effects of MT on tau aggregates in L1 mice. (a) Representative photomicrographs of WT and L1 brain sections, labelled with mAb 7/51, following treatment with MTC as indicated. Both the hippocampus and the cortex were free from tau aggregates in WT mice treated with 45 mg/kg MTC, whereas there were numerous tau-reactive neurons in L1 mice treated with either vehicle or 5 mg/kg MTC. Less tau-positive immunoreactivity was visible in L1 mice treated with 45 mg/kg MTC. Scale bar, 100 μm. The effect size, expressed as ln(count+1) +S.E., is shown for the mean value over all regions and in the hippocampus for (b) MTC and (c) LMTX (in this case, LMTB). *P<0.05, **P<0.01, ***P<0.001. The percentage reduction in counts compared with zero dose is shown at foot. The 45 mg/kg doses are equivalent to 35.1 mg MT/kg and 28.1 mg MT/kg for MTC and LMTB, respectively. L1, Line 1; LMTX, leucomethylthioninium salt; MT, methylthioninium; MTC, methylthioninium chloride; WT, wild type.