Fig. 5.

RotaRod study design and weight charts for L66 mice. (a) A RotaRod test in week 1 was used for cohort matching, followed by 2 weeks of daily drug treatment (Monday–Sunday) and a second RotaRod training (week 4). Tissue was harvested during week 5. In (b) L66 and (c) WT mice, MTC and LMTX (in this case, LMTB) induced a similar, yet significant, dose-dependent weight loss compared with vehicle controls (see asterisks). Values are expressed as mean±SE; *P<0.05. The 45 mg/kg doses are equivalent to 33 mg MT/kg for both MTC and LMTB. L66, Line 66; LMTX, leucomethylthioninium salt; MT, methylthioninium; MTC, methylthioninium chloride; WT, wild type.