Table 1. Primer Sequences.
| Primer name | Assay | Orientation | Sequence (5’– 3’) |
|---|---|---|---|
| F-ABL-A2 | Human DNA control | Forward | TGA CTT TGA GCC TCA GGG TCT GAG TGA AGC C |
| R-ABL-A3 | Human DNA control | Reverse | CCA TTT TTG GTT TGG GCT TCA CAC CAT TCC |
| IAP-F | Mouse DNA | Forward | ATA ATC TGC GCA TGA GCC AAG G |
| IAP-R | Mouse DNA | Reverse | AGG AAG AAC ACC ACA GAC CAG |
| PERT-RT2F | PERT | Forward | TCC TGC TCA ACT TCC TGT CGA G |
| PERT-RT1R | PERT | Reverse | CAC AGG TCA AAC CTC CTA GGA ATG |
| XMRV out F* | MLV diagnostic PCR | Forward | CCG TGT TCC CAA TAA AGC CT |
| XMRV out R | MLV diagnostic PCR | Reverse | TGA CAT CCA CAG ACT GGT TG |
| XMRV in F | MLV diagnostic PCR | Forward | GCA GCC CTG GGA GAC GTC |
| XMRV in R | MLV diagnostic PCR | Reverse | CGG CGC GGT TTC GGC G |
| XMLV-650F | sequencing | Forward | ATG GGA CAG ACC GTA ACC AC |
| XMLV-1200F | sequencing | Forward | CCA GGC ATT CCC ACT CCG CAT |
| XMLV-1300F | sequencing | Forward | TGA AGA TCC AGG TAA ATT GAC TGC C |
| XMLV-1700F | sequencing | Forward | GAG ACT CAA GGA GGC CTA TCG |
| XMLV-2500R | sequencing | Reverse | CAG TCT GGC TTC CTG TGA CAT |
| XMLV-2700F | sequencing | Forward | TTC GCC AAG CGC CTC TGA TTA |
| XMLV-3400F | sequencing | Forward | ACG GCA GGC TTC TGT CGC CT |
| XMLV-3400R | sequencing | Reverse | AGG CGA CAG AAG CCT GCC GT |
| XMLV-3900F | sequencing | Forward | CCA TGC AGT AGA GGC ACT GGT |
| XMLV-4077F # | sequencing | Forward | GAT TGC CTC GAG ATC TTG GC |
| XMLV-4096R* | sequencing | Reverse | GCC AAG ATC TCG AGG CAA TC |
| XMLV-4700F | sequencing | Forward | CTG GGA GCC ACC TAT AAT CAG AT |
| XMLV-5200F | sequencing | Forward | CAG GTA AGT CAT TCG GTG GCC |
| XMLV-5900F | sequencing | Forward | CAG CCC TCA CCA GAT CTT CAA TG |
| XMLV-6800R | sequencing | Reverse | GGG TCA GCT TGT GTT GGG AGG |
| XMLV-7400R | sequencing | Reverse | AGT GTG GTC CGC GTA GAA AC |
| XMLV-7450F | sequencing | Forward | GAA CCG GAG AGG ATT AGA TCT GC |
| XMLV-7900F | sequencing | Forward | AAG AAC CGA TGG TGC CCA CCT |
| XMLV-8161R # | sequencing | Reverse | CGA CTC AGT CTA TCG GAG GA |
| XPR1-out-F | Receptor PCR | Forward | CAC TGG TGT TAC TAC GCG T |
| XPR1-out-R | Receptor PCR | Reverse | GCA ACA AAG TTG TAG AGG T |
* Primers used for preparing 5´-half of the MLV genome for sequencing
# Primers used for preparing 3´-half of the MLV genome for sequencing