Table 2. MLV-positive cell lines tested by nested PCR and PERT-PCR assays.
Cell line | Cell type | MLV-PCR | control PCR | PERT-PCR | |||
---|---|---|---|---|---|---|---|
1st rd | 2nd rd | ABL | IAP | Mg2+ | Mn2+ | ||
22RV1 | prostate cancer | + | + | + | - | + | + |
AC-1M46 | choriocarcinoma-trophoblast hybrid | - | + | + | - | (+) | + |
BT-B | cervix carcinoma (subclone of HELA) | + | + | + | - | - | + |
CML-T11 | T-cell leukemia | - / - | + / - | + / + | + / - | - | - |
COLO-677 | small cell lung carcinoma | + | + | + | - | (+) | + |
DEL | malignant histiocytosis | + | + | + | - | - | + |
EVSA-T | breast carcinoma | + | + | + | - | + | + |
HN1 | oral squamous carcinoma | - / - | + / - | + / + | + / - | n.d. | n.d. |
KARPAS-1106P | B-cell non-Hodgkin lymphoma | + | + | + | - | - | - |
KELLY | neuroblastoma | - | + | + | - | + | + |
KYSE-70 | esophageal carcinoma | + | + | + | - | + | + |
LAMA-87 | chronic myeloid leukema | + | + | + | - | + | + |
LCL-HO | B-lymphoblastoid cells | (+) | + | + | - | + | + |
LXF-289 | lung adenocarcinoma | + | + | + | - | + | + |
M07e1 | acute megakaryoblastic leukemia | + / - | + / - | + / + | + / - | n.d. | n.d. |
ML-1 | follicular thyroid carcinoma | - | + | + | - | + | + |
NAMALWA.CSN/70 | Burkitt lymphoma (subclone) | + | + | + | - | + | + |
NAMALWA.KN2 | Burkitt lymphoma (subclone) | + | + | + | - | + | + |
NCEB-1 | mantle cell lymphoma | + | + | + | +* | - | + |
SCLC-21H | small lung cell carcinoma | + | + | + | - | + | + |
SK-GT-2 | gastric fundus carcinoma | + | + | + | - | + | + |
SK-MEL-1 | melanoma | - | + | + | - | - | - |
SPI-8011 | chronic myeloid leukemia (K-562) | (+) / - | + / - | + / + | + / - | - | - |
* The NCEB-1 cell line is known to harbor several mouse chromosomes in the nuclei of the human cells;-, negative; (+), weakly positive; +, positive; 1human genomic DNA samples contaminated with mouse derived DNA (new DNA preparations revealed MLV and IAP negative and ABL positive results). n.d. not determined.