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A) Representative 2D image from the SBFSEM dataset showing extrasynaptic mitochondria in blue and presynaptic mitochondria in green. B) 3D reconstructions of presynaptic and extrasynaptic mitochondria using TRAKEM2 software. Scale bar = 1000 nm. C) Bar graph depicting the volume of extrasynaptic and presynaptic mitochondria. Data are plotted as mean ± SEM. n = 3 different sets.
