Fig. 1.

Effect of CR on the expression of Cox6b1 mRNA and mitochondrial proteins. a Cox6b1 mRNA expression in the liver of 12-month-old mice fed AL or 30 % CR was determined by qPCR. The mRNA expression of β-actin was used as an internal control. The results are presented as means ± SEM (n = 6, *p < 0.05). b Immunoblotting analysis of mitochondrial subunits using the indicated antibodies. c Densitometry of the immunoblots. The complex II subunit (30 kDa), SDHB, was immunodetected as a loading control. The results are presented as means ± SEM (n = 3, *p < 0.05)