FIGURE 3.

a) Nintedanib inhibits platelet-derived growth factor (PDGF)-BB-stimulated platelet-derived growth factor receptor (PDGFR)-α and β autophosphorylation and proliferation of primary human lung fibroblasts (HLFs). HLFs were incubated with nintedanib at different concentrations and stimulated with PDGF-BB (50 ng·mL⁻¹). PDGFR-α and β phosphorylation was determined by an ELISA specific for the phosphorylated receptors. Proliferation was determined by bromodeoxyuridine incorporation. Concentration-dependent inhibition data are presented as mean±sem (n=3 experiments). Reproduced from [78] with permission from the publisher. b) Nintedanib inhibits basic fibroblast growth factor (FGF) and PDGF-BB-stimulated motility of primary HLF from patients with idiopathic pulmonary fibrosis (IPF-HLF) and without IPF (N-HLF). Human lung fibroblasts were incubated with nintedanib at different concentrations for 30 min before the cells were stimulated with basic FGF (20 ng·mL⁻¹) or PDGF-BB (50 ng·mL⁻¹). Motility of the cells was determined by time lapse microscopy using a Cell-IQ imager (CM Technologies Oy, Tampere, Finland) and manual single cell tracking for 72 h. Mean cell velocity was calculated from three experiments. Inhibition is presented as mean±sem.