(A) Western blotting for FADD, Cyclin D1 and β-Actin in lysates from Luc, KLuc and KFLuc MEFs. (B and C) Alamar blue proliferation assay in cultures of Luc, KLuc and KFLuc MEFs that were either (B) untreated or (C) treated with DMSO, 250 μM CKI-7, 10 μM lonafarnib, or 200 nM PD0325901. (D) Agar colony formation of KLuc, KFLuc, and FADD-reconstituted KFLuc MEF cells treated as indicated for 2 weeks, concentrations as in (C). (E) Fluorescence-activated cell sorting (FACS) analysis of cell cycle distribution in KLuc and KFLuc MEFs 24 hours after the indicated treatment, concentrations as in (C). (F) Western blotting for the indicated proteins in KLuc MEFs treated for 6 hours as in (C). Blots (A and F) and FACS data (E) are representative of 3 independent experiments. Data are means ± SEM from 3 independent experiments.