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. 2015 May 1;6(1):88. doi: 10.1186/s13287-015-0065-6

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© Tsai et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of recombinant endothelin-1 (ET1) on regulation of pre-differentiated human mesenchymal stem cells (hMSCs). (A) Proliferation of hMSCs treated with or without different doses of ET1 was analyzed by quantifying total DNA content. (B) Relative mRNA expression levels of potency- and lineage-related markers in hMSCs treated with or without different doses of ET1 for two passages were analyzed by quantitative RT-PCR. *P < 0.05; n = 3. (C) Growth curves of hMSCs treated with or without 0.1 μM ET1 were generated by calculating cumulative population doublings. (D) Expression of surface antigens of hMSCs treated with or without 0.1 μM ET1 for two passages was assessed by flow cytometry. Area under black curve: cells labeled with target antibody; area under gray curve: cells labeled with isotype antibody.