Table 1.
Primer sequences for quantitative RT-PCR analysis
| Gene name | Accession number | Primer sequences (5′ to 3′) |
|---|---|---|
| OCT3/4 | NM_002701.4 | F: TGGAGAAGGAGAAGCTGGAGCAAAA |
| R: GGCAGATGGTCGTTTGGCTGAATA | ||
| NANOG | NM_021865.2 | F: GCTGAGATGCCTCACACGGAG |
| R: TCTGTTTCTTGACCGGGACCTTGTC | ||
| CBFA1 | NM_004843.3 | F: GGTTCCAGCAGGTAGCTGAG |
| R: AGACACCAAACTCCACAGCC | ||
| SOX9 | NM_000346.3 | F: TAAAGGCAACTCGTACCCAA |
| R: ATTCTCCATCATCCTCCACG | ||
| PPARG2 | NM_138711.3 | F: ATGACAGCGACTTGGCAATA |
| R: GGCTTGTAGCAGGTTGTCTT | ||
| ET1 | NM_001168319.1 | F: AAGGCAACAGACCGTGAAAAT |
| R: CGACCTGGTTTGTCTTAGGTG | ||
| ALP | NM_000478.3 | F: CAAAGGCTTCTTCTTGCTGG |
| R: GGTCAGAGTGTCTTCCGAGG | ||
| OC | NM_199173.3 | F: GACTGTGACGAGTTGGCTGA |
| R: GGAAGAGGAAAGAAGGGTGC | ||
| AGN | NM_013227.2 | F: CACGATGCCTTTCACCACGAC |
| R: TGCGGGTCAACAGTGCCTATC | ||
| COL2 | NM_001844.4 | F: CCTCTGCGACGACATAATCT |
| R: CTCCTTTCTGTCCCTTTGGT | ||
| LPL | NM_000237.2 | F: AGGAGCATTACCCAGTGTCC |
| R: GGCTGTATCCCAAGAGATGGA | ||
| UBC | NM_021009.4 | F: TGAAGACACTCACTGGCAAGACCA |
| R: CAGCTGCTTTCCGGCAAAGATCAA |
Forward and reverse primers are indicated as “F” and “R”, respectively.