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Plots showing real-time PCR amplification using custom TaqMan® Gene Expression Assays. Samples from three Saimiri sciureus (S8, S36 and S34) and one healthy human donor (HUM) were cultured in absence (BR) or presence of PMA/ionomycin (PMA ION) for 12 h, 24 and 48 h. Delta Rn versus cycle amplification plots were obtained using primers and probes for (A) IL-4, (B) IL-5, (C) IL-6, (D) LTA, and (E) IFN-γ. The lines represent the threshold.
