Table 2.
Commercially available human-specific antibodies tested for reactivity with Saimiri cytokines in ICS assays
| Reagents | Clones | Suppliers | References | Human | Saimiri |
|---|---|---|---|---|---|
| IFN-γ capture | mAb GZ-4 | Mabtech | mAb GZ-4 | + | + |
| IFN-γ biotin | mAb 7-B6-1 | Mabtech | mAb 7-B6-1 | + | + |
| IFN-γ capture | B27 | BD Bioscience | 18891D | + | - |
| IFN-γ biotin | 4SB3 | BD Bioscience | 554550 | + | - |
| IL-4 capture | 8D4-8 | BD Bioscience | 554515 | - | - |
| IL-4 biotin | MP4-25D2 | BD Bioscience | 18502D | - | - |
| IL-5 capture | TRFK5 | BD Bioscience | 554393 | + | - |
| IL-5 biotin | JES1-5A10 | BD Bioscience | 554491 | + | - |
| IL-10 capture | JES3-9D7 | BD Bioscience | 554497 | - | - |
| IL-10 biotin | JES3-12G8 | BD Bioscience | 554499 | - | - |
| IL-10 capture | 945A5D11 | Biosource | AHC8102 | - | - |
| IL-10 biotin | 945A5A10 | Biosource | AHC7109 | - | - |
| IL-13 capture | mAb IL13-I | Mabtech | 3470-3 | + | - |
| IL-13 biotin | mAb IL13-II | Mabtech | 3470-6 | + | - |
| TNF capture | mAb1 | BD Bioscience | 554509 | + | - |
| TNF biotin | mAb11 | BD Bioscience | 18642D | + | - |