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. 2015 Apr 8;16(1):73. doi: 10.1186/s13059-015-0639-8

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© Voigt et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overview of the study design. This variability assessment was subdivided into five sub-studies. The comparison of technical variability due to fecal preservation methods and stool homogeneity was addressed in sub-studies 1 and 2, respectively. The conditions for the different preservation methods and the numbers of replicates taken are described for these sub-studies. Sub-study 3 was about the temporal variability within and between subjects. One subject underwent antibiotics treatment (sub-study 4) and bowel cleanse (sub-study 5) in the time course of the study. The sampling time points and number of subjects that collected fecal samples are indicated in the timeline.