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. 2015 Apr 8;16(1):73. doi: 10.1186/s13059-015-0639-8

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© Voigt et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of methods for fecal sampling. (A,B) The samples collected in sub-studies 1 (d₇) and 2 (d₃₉₂) clustered by subject (A) and time point (B) but not according to the preservation method (frozen, RNALater (+4 to 10°C or RT for 1 week (1w) on d₇; frozen and RNALater +4°C, 24 h on d₃₉₂) applied (complete linkage clustering based on Euclidean distance). (C) Shannon diversity index, richness and evenness are shown for d₇ (upper panels) and d₃₉₂ (lower panels) and statistically significant differences are indicated by asterisks with P-value ≤0.05 (unpaired Wilcoxon test).