Figure 5.

The DVC1-p97 Complex Promotes Extraction of the ID Complex from Sites of DNA Damage

(A) U2OS cells cotransfected with FLAG-DVC1 and p97-Myc WT or ATPase-dead (EQ) were treated with MMC (0.3 μM) for 24 hr and then pre-extracted, fixed, and immunostained with indicated antibodies. Scale bar represents 10 μm.

(B) U2OS cells were transfected with control (CTRL) or DVC1 siRNA, treated with HU for 24 hr, pre-extracted, fixed, immunostained, and analyzed by QIBC. A representative image is shown for chromatin-bound FANCI.

(C) Representative plot from QIBC analysis from (B).

(D) Quantification of data from (B). Data represent mean ± SEM from two biologically independent experiments.

(E) Colony formation assay using HeLa cells transfected with indicated siRNAs and subjected to various doses of MMC for 24 hr. Data represent mean ± SEM from two independent experiments using technical triplicates per datapoint.

See also Figure S5.