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A schematic diagram showing the 4 steps adopted in the internal amplification control (IAC) construction. (1) Setting up Inv PCR with 2 inverse primers (Inv P-F and Inv P-R). (2) Restriction digestion (RD) step. (3) Re-ligation step forming new construct with a target insert of ≈375 bp with Cry-9 or Cry-15 primers at each end. (4) Plasmid transformation, insert verification, and storage step.
