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Ethidium bromide-stained 1% agarose gel showing amplification products of the PCR assay using 10-fold serial dilutions of IAC recombinant DNA in duplex reactions with the EAC. M, GeneRulerTM 100 bp DNA marker; Lane 1, EAC/IAC (0.4 fg, 20 fg); Lane 2, (0.4 fg, 10 fg); Lane 3, (0.4 fg, 1 fg); Lane 4, (0.4 fg, 0.5 fg); Lane 5, (0.4 fg, 0.2 fg). The optimum concentration of IAC in the PCR assay (encircled) was 1 fg per reaction.
