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. 2014 Apr 9;23(3):426–446. doi: 10.1017/S0967199414000033

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© Cambridge University Press 2014

The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution licence <http://creativecommons.org/licenses/by/3.0/>.

PMC Copyright notice

LiCl, PKC activator and PKC inhibitors affect the distribution of β-catenin. Localization of β-catenin (green) and nuclear staining (red) in the H. pulcherrimus embryos at the 16-cell, 28-cell and 56-cell stages. (A) Control embryos. (B) Embryos treated with 40 mM LiCl starting from the 4-cell stage. (C) Embryos exposed to PKC activator (8 nM PMA for 20 min starting from 5 min before the 4th cleavage). (D, E) Embryos exposed to 5 μM HpPKC-I starting from 20 min before the 4th division (16-cell stage). (F) Embryos exposed to 400 nM Gö6976, a calcium-dependent PKC inhibitor, for the same period as HpPKC-I. Abbreviations: An, animal side; Vg, vegetal side; mic; micromeres, Mac; macromeres, meso; mesomeres.