Figure 8.

Morphological changes of H. pulcherrimus embryos treated with HpPKC inhibitor before the 8-cell stage. (A) Control embryos. (B) Embryos treated with 5 μM HpPKC-I starting from the 4-cell stage (20 min before the 3rd cleavage) to the end of 8-cell stage. (C) Embryos treated with 5 μM HpPKC starting from 20 min before the 4th cleavage to the end of 16-cell stage. Embryos were cultured at 18°C. The hand-drawn delineations on the right panels represent the embryos marked with asterisks in (A–C). Oral ectoderm area and aboral area were indicated by white arrowheads and black arrows, respectively.