Table 1.
Treatments | Micromeres | Macromeres | Mesomeres |
---|---|---|---|
Control | 0.99 ± 0.02 (24) | 0.91 ± 0.01 (23) | 0.92 ± 0.02 (33) |
5 μM HpPKC-I | 0.91 ± 0.02 (17) | 0.89 ± 0.02 (16) | 0.90 ± 0.02 (26) |
400 nM Gö6976 | 1.06 ± 0.02 (5) | 0.98 ± 0.03 (5) | 0.96 ± 0.02 (7) |
8 nM PMA | 0.76 ± 0.00 (2) | 0.72 ± 0.04 (2) | 0.69 ± 0.03 (2) |
Ca2+ levels of each blastomere were reported as mean ± standard deviation (SD) of the ratios of fluorescence intensity of Ca2+-sensitive dye (calcium green BAPTA) to the intensity of the Ca2+-insensitive internal control dye (rhodamine red) in the same cell. The numbers of monitored cells were given in parentheses.