Table 2.
Effects of PKC modulators on the formation of the skeletal rods in the pluteus (P. lividus)
| Body rods (Br) | Post-oral rods (Por) | Antero-lateral rods (Alr) | Total length (μm) | |
|---|---|---|---|---|
| Control | 103.5 ± 0.95 (102) | 51.1 ± 0.90 (115) | 64.0 ± 0.67 (118) | 219 |
| HpPKC-I | 91.5 ± 0.67 (132) | 42.2 ± 0.90 (118) | 63.8 ± 0.67 (128) | 198 |
| Gö6976 | 103.2 ± 0.53 (163) | 61.2 ± 0.59 (145) | 75.9 ± 0.73 (146) | 240 |
| PMA | 103.5 ± 0.39 (129) | 55.9 ± 0.76 (125) | 68.8 ± 0.56 (130) | 228 |
| LiCl | 101.2 ± 0.71 (85) | 45.4 ± 0.72 (77) | 56.5 ± 0.99 (72) | 203 |
In total, 5 μM HpPKC-I and 400 nM Gö6976 were added to embryos 20 min before the 4th cleavage; 8 nM PMA was added to the 16-cell stage embryos for 20 min, and 40 mM LiCl to the embryos at the 4-cell to 60-cell stages (2.5 h). The numbers of monitored cells are given in parentheses.