Table 3.
Gastrula | Pluteus | ||
---|---|---|---|
Genes | 20 h | 23 h | 43 h |
Endomesodermal transcription factors and signal components | |||
Delta | NS | NS | NS |
Gcm | −2.73 | NS | NS |
Fox A | NS | NS | NS |
Bra | NS | NS | NS |
Blimp/Krox | NS | NS | +1.84 |
Brain1/2/4 | −4.78 | −3.91 | NS |
PMC ingression and skeletogenic genes | |||
G-cadherin | NS | −1.81 | NS |
Sm50 | −2.71 | −5.26 | NS |
Sm37 | −2.40 | NS | NS |
Sm30 | −2.34 | −1.92 | NS |
Regulator genes of O–A axis formation | |||
Nodal | +3.81 | NS | NS |
Deadringer (Dri) | −5.44 | −4.00 | +3.32 |
Lim | NS | NS | +3.22 |
Gsc | −2.02 | −2.46 | NS |
FoxG | −1.95 | −1.89 | NS |
NK2.2 | −4.40 | −1.70 | NS |
Coquilette | −2.53 | −1.79 | NS |
Embryos were treated with 5 μM HpPKC-I starting from 20 min before the 4th cleavage until 20 min after the 6th cleavage. Embryos were harvested at time points 20 h (early gastrula), 23 h (late gastrula or prism) and 43 h (two-armed pluteus) post fertilization. The D values defined in Methods and materials were calculated from the results of qPCR and reported in positive and negative numbers that respectively represent increases and decreases of the transcripts after the treatment with the inhibitor. NS, not significant (P > 0.05).