Table 3.

qPCR analysis of embryos treated with HpPKC inhibitor

	Gastrula	Pluteus
Genes	20 h	23 h	43 h
Endomesodermal transcription factors and signal components
Delta	NS	NS	NS
Gcm	−2.73	NS	NS
Fox A	NS	NS	NS
Bra	NS	NS	NS
Blimp/Krox	NS	NS	+1.84
Brain1/2/4	−4.78	−3.91	NS
PMC ingression and skeletogenic genes
G-cadherin	NS	−1.81	NS
Sm50	−2.71	−5.26	NS
Sm37	−2.40	NS	NS
Sm30	−2.34	−1.92	NS
Regulator genes of O–A axis formation
Nodal	+3.81	NS	NS
Deadringer (Dri)	−5.44	−4.00	+3.32
Lim	NS	NS	+3.22
Gsc	−2.02	−2.46	NS
FoxG	−1.95	−1.89	NS
NK2.2	−4.40	−1.70	NS
Coquilette	−2.53	−1.79	NS

Embryos were treated with 5 μM HpPKC-I starting from 20 min before the 4th cleavage until 20 min after the 6th cleavage. Embryos were harvested at time points 20 h (early gastrula), 23 h (late gastrula or prism) and 43 h (two-armed pluteus) post fertilization. The D values defined in Methods and materials were calculated from the results of qPCR and reported in positive and negative numbers that respectively represent increases and decreases of the transcripts after the treatment with the inhibitor. NS, not significant (P > 0.05).