Table 4.
Primer properties
| Name | Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size [bp] | Concentration [nM] | Efficiency |
|---|---|---|---|---|---|---|
| Calmodulin (HKG) | cal | TGATGGCGCGCAAGATGAAGG | TGCCATCGCGATCGAAAACCTTG | 78 | 750 | 98% |
| Cytochrome oxidase subunit 1 (HKG) | cox | TCTGTCTTCCTCTCACATCTCT | CCACTGCACCATTTCCAAGA | 82 | 225 | 97% |
| S-adenosyl methionine synthetase (HKG) | sam | GACCAAGAACGGCATCAAGT | TGCTGCTCATGGATGCATAC | 74 | 200 | 95% |
| ß-tubulin (HKG) | tub | CCAGCTTTGCCATTCCCTTG | TGGTTCCACCACTGTGTCAG | 148 | 750 | 94% |
| Hybrid ascorbate-cytochrome c peroxidase (GOI) | apx1 | CAATGTGGCACTCATGCTGG | TAAGCTTCTCAAGGTCCGCC | 107 | 500 | 102% |
| Catalase peroxidase (GOI) | katg1 | TCTTCTTGGCCAAGTGAAGC | TTTGATGGCAGTGGTTCCTG | 85 | 500 | 96% |
| Manganese superoxide dismutase (GOI) | mnsod1 + 2 | CAACCCCAAACCAGGACAAT | CACATCCCACCAAGCTTTGA | 146 | 1000 | 96% |
Primer sequences, amplicon size, equimolar primer concentrations and efficiency for housekeeping genes (HKGs) and antioxidant genes of interest (GOI) used for quantitative real-time PCR-based (SYBR® Green) gene expression in Symbiodinium type B1. The isoform designations for the antioxidant genes are based on results presented in this study.