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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Methods Mol Biol. 2015;1239:105–132. doi: 10.1007/978-1-4939-1862-1_6
5 × Phusion HF Buffer 10 μl
10 mM dNTPs mix 1 μl
Phusion High-Fidelity DNA polymerase 0.5 μl
10 pmol/μl PCR primers used in Subheading 3.3.2, Step 28 2.5 μl each
Purified plasmids 1 μl
Water 32.5 μl