| PURExpress solution A | 10 μl |
| PURExpress solution B | 7.5 μl |
| Murine RNase inhibitor (40 units/μl) | 0.5 μl |
| Gel-extracted library (4 ng/μl) | 2 μl |
| 10 mg/mL BSA | 3 μl |
| Water | 7 μl |
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