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Experimental design of the three studies to assess the impact of post-weaning social isolation on approach behavior induced by pro-social 50-kHz USV. (A) Experimental designs: Experiment 1: Post-weaning Social Isolation—Effects; Experiment 2: Post-weaning Social Isolation—Rescue; and Experiment 3: Post-adolescent Social Isolation—Effects. (B) Schematic illustration of the rat developmental profile, highlighting the rough-and-tumble play period.
